Evaluation of modified passive haemagglutination assay for Vi antibody estimation in Salmonella typhi infections.
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چکیده
منابع مشابه
Evaluation of modified passive haemagglutination assay for Vi antibody estimation in Salmonella typhi infections.
A simple passive haemmagglutination assay (PHA) was developed to detect Vi antibodies, to improve the diagnosis of typhoid fever by small laboratories. The Vi capsular antigen of Salmonella typhi was extracted by alternate alcohol and acetone precipitation. Formalin fixed, sheep red blood cells treated with chromium chloride were sensitised with this Vi antigen and antibodies detected and measu...
متن کاملEvaluation of a new assay for Vi antibody in chronic carriers of Salmonella typhi.
The assay for serum antibody to the Salmonella typhi capsular polysaccharide (Vi) antigen has recently been revised because of the availability of a purified, highly polymerized Vi antigen. We compared this revised Vi antibody assay to the traditional one for potential usefulness in the surveillance of chronic enteric carriers of S. typhi. The purified Vi antigen of Citrobacter freundii was inc...
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متن کاملEnzyme-linked immunosorbent assay for detection of Salmonella typhi Vi antigen in urine from typhoid patients.
Because typhoid fever continues to be a major cause of illness in many developing countries, there is a clear need for a sensitive and specific test that will permit rapid laboratory diagnosis of the disease. An enzyme-linked immunosorbent assay (ELISA) has recently been developed and tested, both in the laboratory and in a clinical situation, for its ability to detect Vi antigen in urine. The ...
متن کاملDevelopment and evaluation of an enzyme-linked immunosorbent assay for serum Vi antibodies for detection of chronic Salmonella typhi carriers.
An enzyme-linked immunosorbent assay (ELISA) measuring, in serum, immunoglobulin G (IgG), IgM, and IgA to Vi capsular polysaccharide antigen that was tyraminated (Vi-Tyr) to increase its binding efficiency to microtiter plates was compared with the standard passive hemagglutination assay (PHA) as a screening test for chronic Salmonella typhi carriers. Initially, three populations were evaluated...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1992
ISSN: 0021-9746
DOI: 10.1136/jcp.45.8.740